Innovative MRI techniques in neuroimaging approaches for cerebrovascular diseases and vascular cognitive impairment

Cognitive impairment and dementia are recognized as major threats to public health. Many studies have shown the important role played by challenges to the cerebral vasculature and the neurovascular unit. To investigate the structural and functional characteristics of the brain, MRI has proven an invaluable tool for visualizing the internal organs of patients and analyzing the parameters related to neuronal activation and blood flow in vivo. Different strategies of imaging can be combined to obtain various parameters: (i) measures of cortical and subcortical structures (cortical thickness, subcortical structures volume); (ii) evaluation of microstructural characteristics of the white matter (fractional anisotropy, mean diffusivity); (iii) neuronal activation and synchronicity to identify functional networks across different regions (functional connectivity between specific regions, graph measures of specific nodes); and (iv) structure of the cerebral vasculature and its efficacy in irrorating the brain (main vessel diameter, cerebral perfusion). The high amount of data obtainable from multi-modal sources calls for methods of advanced analysis, like machine-learning algorithms that allow the discrimination of the most informative features, to comprehensively characterize the cerebrovascular network into specific and sensitive biomarkers. By using the same techniques of human imaging in pre-clinical research, we can also investigate the mechanisms underlying the pathophysiological alterations identified in patients by imaging, with the chance of looking for molecular mechanisms to recover the pathology or hamper its progression

Porokeratosis: Two Faces, One Family

Porokeratosis is a disorder of keratinisation whose pathogenesis is yet unclear. It has been postulated that it results from the proliferation of an abnormal clone of keratinocytes, triggered by several factors, such as immunosuppression or prolonged ultraviolet exposure. Various clinical forms are recognized whose common denominator is a keratotic ring surrounding a central zone of atrophy. The histological hallmark is the cornoid lamella, a thin column of hyperproliferative abnormal keratinocytes. We describe two cases of porokeratosis. A 67-year-old woman with an erythematous purplish round plaque surrounded by a keratotic border that had appeared 6 years previously on the left sural region was diagnosed as ‘giant’ porokeratosis. A 49-year-old man presented with small papules coalescent in an erythematous oval plaque on the lateral side of the left foot consistent with linear porokeratosis

Widely distributed photonics-based dual-band MIMO radar for harbour surveillance

A new architecture for a widely distributed dual-band coherent multiple-input multiple-output (MIMO) radar system is illustrated, and its implementation and testing are reported. The system consists in a central unit where radar signals are coherently generated and detected, which serves multiple remote sensors connected over transparent WDM optical network. Every remote node operates coherently both in the S- and X-band, and is displaced over distances of several kilometers, allowing to monitor a scene under different angles of view. All the remote sensors share the same oscillator and digital signal processing unit, both located in the central office, allowing to perform centralized raw data fusion on the acquired signals. By virtue of the system coherence, the system takes advantage of the coherent MIMO processing strategy to offer a superior spatial resolution, which is even magnified by the dual-band approach

Distributed coherent radars enabled by fiber networks

In the last few years, we have been proposing the use of photonics to bring new functionalities in radar systems, exploiting its precision and tunability to give radars improved performance and reconfigurability. This paper will present the most recent evolution of the original idea of photonics-based radars, which considers the exploitation of the increasingly available fiber connections to implement a network of widely distributed radars. The centralized photonic approach allows driving several separated radars simultaneously, enabling the novel class of distributed coherent radar systems. Through the implementation of specific multi-input multi-output (MIMO) processing, the photonics-enabled radar network is capable to push the radar detection resolution far beyond the limits usually set by the signal bandwidth alone

Photonics for Coherent MIMO Radar: an Experimental Multi-Target Surveillance Scenario

This paper investigates the target detection and localization capabilities of a coherent multiple input multiple output (MIMO) radar network designed and implemented using photonic technology. The benefit offered by photonics is twofold: it guarantees long-time phase stability and frequency/phase coherence between the transmitted and received radio frequency signals; secondly, it allows remoting the antennas by exploiting optical fibers. The proposed radar network demonstrator, which is composed of two transmitting and two receiving antennas in the X-band with 100 MHz signal bandwidth, operates in a real down-scaled outdoor scenario for detecting two collaborative closely-spaced moving targets. The preliminary results demonstrate the effective impact of photonics applied to coherent centralized radar networks and provide some guidelines for the development of more complex and application-tailored radar networks

In-Field Demonstration of a Photonic Coherent MIMO Distributed Radar Network

This paper reports an in-field experiment of a photonics-based coherent MIMO radar network. The use of photonics guarantees the coherence of the transmitted and received RF signals, and allows remoting the antennas exploiting deployed optical fibers, thus a MIMO approach can be applied on a network of widely distributed coherent radars. In the in-field experiment, a photonics-based radar core connects two transmitters and two receivers, with 100-MHz bandwidth signals in X-band, observing a collaborative target. The results demonstrate an improvement in radar precision, and envisage real applications wherever fiber is available for deploying the radar network

Coherent dual-band 2x4 MIMO radar experiment exploiting photonics

In this paper the first coherent dual-band 2x4 MIMO radar experiment is presented. Range/cross-range maps demonstrate the higher cross-range resolution due to the coherence and the enhanced performance introduced by dual-band operatio

Elucidating the phylodynamics of endemic rabies virus in eastern Africa using whole-genome sequencing

Many of the pathogens perceived to pose the greatest risk to humans are viral zoonoses, responsible for a range of emerging and endemic infectious diseases. Phylogeography is a useful tool to understand the processes that give rise to spatial patterns and drive dynamics in virus populations. Increasingly, whole-genome information is being used to uncover these patterns, but the limits of phylogenetic resolution that can be achieved with this are unclear. Here, whole-genome variation was used to uncover fine-scale population structure in endemic canine rabies virus circulating in Tanzania. This is the first whole-genome population study of rabies virus and the first comprehensive phylogenetic analysis of rabies virus in East Africa, providing important insights into rabies transmission in an endemic system. In addition, sub-continental scale patterns of population structure were identified using partial gene data and used to determine population structure at larger spatial scales in Africa. While rabies virus has a defined spatial structure at large scales, increasingly frequent levels of admixture were observed at regional and local levels. Discrete phylogeographic analysis revealed long-distance dispersal within Tanzania, which could be attributed to human-mediated movement, and we found evidence of multiple persistent, co-circulating lineages at a very local scale in a single district, despite on-going mass dog vaccination campaigns. This may reflect the wider endemic circulation of these lineages over several decades alongside increased admixture due to human-mediated introductions. These data indicate that successful rabies control in Tanzania could be established at a national level, since most dispersal appears to be restricted within the confines of country borders but some coordination with neighbouring countries may be required to limit transboundary movements. Evidence of complex patterns of rabies circulation within Tanzania necessitates the use of whole-genome sequencing to delineate finer scale population structure that can that can guide interventions, such as the spatial scale and design of dog vaccination campaigns and dog movement controls to achieve and maintain freedom from disease